Homoharringtonine exerts anti-silicosis effects by inhibiting the CCR1 and PI3K/AKT signaling pathways in lung fibroblasts

Silicosis is an occupational lung disease caused by prolonged exposure to silica dust in the workplace. It has a complex pathogenesis and currently lacks effective treatments. Homoharringtonine(HHT), a natural compound approved for the treatment of acute myeloid leukemia, but its effects on silicosis are unclear. In the present study, we constructed a mouse model of silica (SiO ₂)-induced pulmonary fibrosis and evaluated the preventive and therapeutic capacity of HHT. The results showed that HHT attenuated the progression of SiO ₂-induced pulmonary fibrosis in mice. We then used MRC-5, a human lung fibroblast cell line to explore the mechanisms underlying HHT's inhibitory effects <i>in vitro</i> and found that HHT significantly inhibited the activation and migratory capacity of MRC-5 cells. Mechanistically, the effects were mediated by enhanced ubiquitination and degradation of the CCR1 protein. Furthermore, HHT exhibited favorable biocompatibility <i>in vivo</i>, and its preventive and therapeutic effects were validated in SiO ₂-treated mice. This study demonstrates that HHT holds significant potential as a therapeutic agent for silicosis by targeting CCR1 and the PI3K/AKT/mTOR signaling pathway, highlighting it as a promising candidate for clinical development in silicosis treatment.