Engineering a Mi3<sub>Chemout</sub> system for controlled recruitment and release of proteins of interest
Protein cages are ideal scaffolds for presenting proteins of interest (POIs), but controlled POI release remains challenging. We engineered the Mi3 protein cage with chemically inducible dimerization domains and a photocleavable protein, enabling controlled recruitment and release of POIs both <i>in vitro</i> and in <i>E. coli</i> cells.
Authors
- Xiao Ma (ORCID: https://orcid.org/0000-0003-2978-8500)
- Wei Kang (ORCID: https://orcid.org/0000-0002-2288-3540)
- Shijie Ren (ORCID: https://orcid.org/0000-0003-3568-7124)
- Hong Yuan
- Chuang Xue (ORCID: https://orcid.org/0000-0002-3856-8457)
- Yongsheng Yu (ORCID: https://orcid.org/0000-0001-5139-3439)
- Chunxue Liu (ORCID: https://orcid.org/0000-0002-8805-8869)
- Lingqin Wang
Institutions
- Dalian University of Technology (CN)
- Dalian University (CN)
- Dalian Municipal Central Hospital (CN)
- Chongqing Institute of Green and Intelligent Technology (CN)
Publication Details
- Journal
- Chemical Communications
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1039/d5cc00808e
- Primary Topic
- Advanced biosensing and bioanalysis techniques
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China
- Natural Science Foundation of Ningbo Municipality