Biases from Oxford Nanopore library preparation kits and their effects on microbiome and genome analysis
Our findings indicated that careful and consistent library preparation method selection is essential for quantitative methods such as bovine-related microbiome analysis due to the systematic bias induced by the enzymatic reactions in Oxford Nanopore library preparation.
Authors
- Elizabeth M. Ross (ORCID: https://orcid.org/0000-0002-7521-3671)
- Mónica Gutiérrez‐Rivas (ORCID: https://orcid.org/0000-0001-9752-7498)
- Sarah J. Meale (ORCID: https://orcid.org/0000-0002-8417-1186)
- Chian Teng Ong (ORCID: https://orcid.org/0000-0003-2432-2509)
- Loan Nguyen (ORCID: https://orcid.org/0000-0002-7783-5466)
- Harrison J. Lamb (ORCID: https://orcid.org/0000-0003-2864-7685)
- Óscar González-Recio (ORCID: https://orcid.org/0000-0002-9106-4063)
- Ziming Chen (ORCID: https://orcid.org/0009-0005-2398-8466)
Institutions
- The University of Queensland (AU)
- Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (ES)
- Agriculture and Food (AU)
- Department of Primary Industries (AU)
Publication Details
- Journal
- BMC Genomics
- Published
- 2025-05-19
- DOI
- https://doi.org/10.1186/s12864-025-11649-z
- Citations
- 9
- Primary Topic
- Microbial Community Ecology and Physiology
- Type
- article
- Field-Weighted Citation Impact
- 15.45
Funders
- Meat and Livestock Australia
- Queensland Alliance for Agriculture and Food Innovation, University of Queensland