Characterization of genome-wide transpositions induced by colistin exposure in multi-drug-resistant <i>Klebsiella pneumoniae</i>
Colistin is one of the last-line antibiotics against multi-drug-resistant (MDR) gram-negative pathogens, such as <i>Klebsiella pneumoniae</i>. Using long-read sequencing, we observed remarkable genome-wide transposition events in MDR <i>K. pneumoniae</i> exposed to colistin in patients receiving treatment for respiratory infections or as part of selective decolonization strategies and further confirmed these on <i>in vitro</i> selection experiments. These data add yet another dimension to the role of antibiotics in mediating specific processes in bacteria beyond antibiotic resistance.CLINICAL TRIALSThis study is registered with ClinicalTrials.gov as NCT02208154.
Authors
- Y. Glupczynski (ORCID: https://orcid.org/0000-0002-9419-6387)
- Basil Britto Xavier (ORCID: https://orcid.org/0000-0002-5897-0240)
- Sahaya Glingston Rajakani (ORCID: https://orcid.org/0000-0002-0239-1418)
- Olympia Zarkotou (ORCID: https://orcid.org/0000-0002-0579-3404)
- Nienke L. Plantinga (ORCID: https://orcid.org/0000-0002-5979-0313)
- Surbhi Malhotra‐Kumar (ORCID: https://orcid.org/0000-0001-8699-8905)
- Qiang Lin (ORCID: https://orcid.org/0000-0002-9916-7761)
- Rob Van Houdt (ORCID: https://orcid.org/0000-0002-7459-496X)
- Bastiaan HJ Wittekamp
- Spyros Pournaras (ORCID: https://orcid.org/0000-0002-6298-9577)
- Anouk J. M. M. Braspenning (ORCID: https://orcid.org/0009-0007-1665-5289)
- Minh Ngoc Nguyen (ORCID: https://orcid.org/0000-0001-7652-1898)
- Marc J. M. Bonten
Institutions
- University of Antwerp (BE)
- National and Kapodistrian University of Athens (GR)
- University Medical Center Utrecht (NL)
- Belgian Nuclear Research Centre (BE)
- Infectious Diseases Institute (UG)
- Tzaneion General Hospital (GR)
- Oklahoma State University Center for Health Sciences (US)
Publication Details
- Journal
- Antimicrobial Agents and Chemotherapy
- Published
- 2025-05-19
- DOI
- https://doi.org/10.1128/aac.01574-24
- Citations
- 1
- Primary Topic
- Antibiotic Resistance in Bacteria
- Type
- article
- Field-Weighted Citation Impact
- 0.59
Funders
- Universiteit Antwerpen