Two new species of Neohelicosporium (Tubeufiaceae, Tubeufiales) from freshwater and terrestrial habitats in China

<i>Neohelicosporium</i> species are a group of helicosporous hyphomycetes with the potential to produce novel bioactive natural compounds. During our investigation on helicosporous hyphomycetes, six isolates were collected from freshwater and terrestrial habitats in the tropical and subtropical regions of southern China. Based on multi-gene phylogenetic analyses (LSU-ITS-<i>tef</i>1-α-<i>rpb</i>2) and morphological comparisons of <i>Neohelicosporium</i> taxa, two new species (<i>N.guizhouense</i> and <i>N.wuzhishanense</i>) are introduced. Detailed micro-morphological descriptions, illustrations, and DNA molecular data are provided for the newly introduced species to confirm their taxonomic placements.