Effect of 25(OH)D supplementation on adipokines and advanced glycation end products in experimental type 2 diabetic and obesity rat model

Diabetes mellitus is a global health problem and vitamin D deficiency is thougt to be a reasonal factor for development of diabetes. Our aim is to investigate among vitamin D levels and blood glucose, insulin resistance, adiponectin, apelin, tumor necrosis factor-alpha (TNF-), interleukin-6 (IL-6), oxidative stress index (OSI) levels and advanced glycation end products (AGEs) formation in modeled obesity and type 2 diabetes mellitus (T2DM) rats. During the 14-week experiment; Control group was fed standart diet-(12 % of calories of fat), Diabetes group was fed high fat diet (HFD)-at the 4^th week Streptozotocin (STZ) (35 mg/kg) was injected, Diabetes/vitamin D group was fed HFD-at the 4^th week STZ was injected-between 4^th- 14^th week vitamin D supplement was administered, Obesity group was fed HFD-(40% of calories as fat), Obesity/vitamin D group was fed HFD-between 4^th-14^th week vitamin D supplement was administered. At 14^th week, biochemical parameters, adipokine concentrations, kidney tissue cytokine levels, liver tissue oxidative stress index parameters were measured by using ELISA method and Hematoxylinamp;Eosin staining of kidney, heart, liver, coronary/renal artery tissues and immunohistochemical staining of kidney tissues were performed. Vitamin D can alleviate glucose and lipid parameters and we think vitamin D may be useful adjuvant agent in obesity and T2DM treatment.