QUANTITATIVE DETERMINATION OF BERBERINE IN THE ABOVEGROUND ORGANS OF MAHONIA AQUIFOLIUM (PURSH) NUTT

Our study's objective was to use high-performance liquid chromatography (HPLC) to quantify the amount of berberine in the total amount of alkaloids extracted from M. aquifolium. Eclipse plus C-18 was used to separate the crude extract (250 x 4.6 x 5 µm). Methanol (0.1% formic acid): water (0.1 formic acid) 35:65 v v-1 made up the mobile phase. At 346 nm, ultraviolet detection was carried out. With a r2 > 0.9998, a solid linear response was seen in the concentration range under study. The alkaloid extract from the aerial parts of M. aquifolium should contain at least 2% berberine, according to the results. With intra- and inter-day variances of less than 1.03%, the approach is accurate. Within the range under study, linearity, sensitivity, and precision are all adequate.