Enhancing infectious intestinal disease diagnosis through metagenomic and metatranscriptomic sequencing of 1000 human diarrhoeal samples
This study highlights the power of directly sequencing nucleic acids from human samples to augment gastrointestinal pathogen surveillance and clinical diagnostics. Metatranscriptomics was most effective for identifying a wide range of pathogens and showed superior sensitivity. We propose that metatranscriptomics should be considered for future diagnosis and surveillance of gastrointestinal pathogens. We assembled a rich data resource of paired metagenomic and metatranscriptomic datasets, direct from patient stool samples, and have made these data publicly available to enhance the understanding of pathogens associated with infectious intestinal diseases.
Authors
- K. Marie McIntyre (ORCID: https://orcid.org/0000-0003-1360-122X)
- Alistair C. Darby (ORCID: https://orcid.org/0000-0002-3786-6209)
- Jay C. D. Hinton (ORCID: https://orcid.org/0000-0003-2671-6026)
- Maya Wardeh (ORCID: https://orcid.org/0000-0002-2316-5460)
- Blanca M. Perez-Sepulveda (ORCID: https://orcid.org/0000-0002-2891-4010)
- Miren Iturriza‐Gómara
- Nigel A. Cunliffe (ORCID: https://orcid.org/0000-0002-5449-4988)
- Yan Li (ORCID: https://orcid.org/0000-0002-7480-9545)
- S. O’Brien (ORCID: https://orcid.org/0000-0003-2896-8999)
- Charlotte A. Nelson
- Christiane Hertz-Fowler
- Sam Haldenby
- Edward Cunningham-Oakes
- Richard Gregory
Institutions
- University of Liverpool (GB)
- Global Vaccines (United States) (US)
- Health Protection Research Unit in Emerging and Zoonotic Infections at University of Liverpool (GB)
- Newcastle University (GB)
Publication Details
- Journal
- Genome Medicine
- Published
- 2025-05-19
- DOI
- https://doi.org/10.1186/s13073-025-01478-w
- Citations
- 1
- Primary Topic
- Viral gastroenteritis research and epidemiology
- Type
- article
- Field-Weighted Citation Impact
- 1.40