Targeted high-level production of chuangxinmycin and its halogenated derivatives with antitubercular activity
Synthetic biology techniques are well-suited for the targeted and high-level biosynthesis of CM and its derivatives. This study reports the highest laboratory-level yields of CM and NCM to date. This is the first instance of obtaining CM derivatives by biosynthesis rather than chemical synthesis, and it also marks the first report of halogenated NCM derivatives. High-level production of CM and its diverse analogues will provide a solid material foundation for advancing CM and its derivatives as potential anti-tuberculosis drug candidates.
Authors
- Bin Hong (ORCID: https://orcid.org/0000-0001-6244-8298)
- Yuanyuan Shi (ORCID: https://orcid.org/0000-0003-2997-4573)
- Xingli Han
- Xingquan Zhao (ORCID: https://orcid.org/0000-0001-8345-5147)
- Tianyu Zhang (ORCID: https://orcid.org/0000-0002-6501-7562)
- Xintong Zhang (ORCID: https://orcid.org/0000-0003-2302-9642)
- Xingxing Li (ORCID: https://orcid.org/0000-0002-6164-8738)
- Yuan Lu (ORCID: https://orcid.org/0000-0002-8046-669X)
- Yu Du (ORCID: https://orcid.org/0000-0002-3263-0141)
- Xiumin Zhang (ORCID: https://orcid.org/0000-0002-3906-1180)
- Linzhuan Wu
- Yuting Zhu (ORCID: https://orcid.org/0000-0001-7412-7272)
- Wei Yu (ORCID: https://orcid.org/0009-0003-3765-418X)
Institutions
- University of Science and Technology of China (CN)
- Chinese Academy of Sciences (CN)
- Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
- Guangzhou Institutes of Biomedicine and Health (CN)
- State Key Laboratory of Respiratory Disease (CN)
- University of Chinese Academy of Sciences (CN)
Publication Details
- Journal
- Microbial Cell Factories
- Published
- 2025-05-19
- DOI
- https://doi.org/10.1186/s12934-025-02740-x
- Primary Topic
- Microbial Natural Products and Biosynthesis
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China
- Beijing Nova Program
- National Key Research and Development Program of China