Development and validation of nanoplate-based RT-dPCR assay for canine respiratory coronavirus detection in various clinical samples
This study demonstrated that the RT-dPCR assay provided high sensitivity for detecting low viral loads across various sample types, making it a valuable tool for precise CRCoV detection. In contrast, RT-qPCR remains valuable for its broader detection range and suitability in initial screening. Both techniques proved to be versatile tools that can contribute to advancing CRCoV research and improving clinical diagnostics.
Authors
- Chutchai Piewbang (ORCID: https://orcid.org/0000-0002-5148-4696)
- Somporn Techangamsuwan (ORCID: https://orcid.org/0000-0002-4888-2677)
- Panida Poonsin (ORCID: https://orcid.org/0000-0003-3010-7071)
- Vorapun Wiwatvisawakorn
Institutions
- Chulalongkorn University (TH)
- Bangkok Hospital (TH)
Publication Details
- Journal
- BMC Veterinary Research
- Published
- 2025-05-17
- DOI
- https://doi.org/10.1186/s12917-025-04807-8
- Primary Topic
- SARS-CoV-2 detection and testing
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Chulalongkorn University
- National Research Council of Thailand
- Thailand Science Research and Innovation