ANTICANCER ACTIVITY OF CURCUMA KARNATAKENSIS – A REAR AND ENDEMIC PLANT USING SULFORHODAMINE-B ASSAY

Objectives: The objectives are to study the anticancer activity of Curcuma karnatakensis collected from different localities using sulforhodamine-B (SRB) assay. Methods: Anticancer activities of two plant samples were detected on human breast cancer cells (MDA-MB-468) by following SRB assay method. We have used two plant samples collected from two different localities, we specifically used 1.5 mg and 3 mg of extract and study was done for 24 h and 48 h duration, optical density was read at 540 nm using microplate reader, and percentage inhibition of cancer cell growth was recorded. The low concertation of the plant sample was found to be very active and effective than the other in less duration of time. Results: Anticancer activity of both the samples was analyzed for different time durations with different concentrations and it was found that sample B has not shown anticancer activity at both the treatments. The difference in their activity may be due to the difference in growing areas and difference in soil type where it is growing with different mineral compositions. Conclusion: The present work on this rare species gives basic information regarding their anticancerous behavior in low dosages and this is the stepping stone to study further about use of this plant in the medicinal field.