Evaluation of plasma nucleosome concentrations and the effect of pre-analytical variables in healthy cats
Nucleosomes can be successfully measured in blood obtained from healthy cats. EDTA tubes provided more consistent results compared to sodium citrate and serum separator tubes for evaluation of H3.1 nucleosome levels. The significant increase in nucleosome concentration in EDTA samples that were processed after 120 min justifies sample processing within one hour of collection. Samples can be processed utilizing any of the centrifugation protocols used in this study.
Authors
- Samuel D. Stewart (ORCID: https://orcid.org/0000-0003-1148-2589)
- Heather Wilson‐Robles (ORCID: https://orcid.org/0000-0002-6489-8468)
- Emma Warry
- Mhammed Bougoussa (ORCID: https://orcid.org/0000-0002-8823-8544)
- Justin Heinz
- Stephanie Istvan
- Emily Westerback
Institutions
- Orthopedic Specialty Hospital (US)
- Ethos (United Kingdom) (GB)
- Volition (United States) (US)
- Volition (Belgium) (BE)
- Texas A&M University (US)
Publication Details
- Journal
- BMC Veterinary Research
- Published
- 2025-05-17
- DOI
- https://doi.org/10.1186/s12917-025-04785-x
- Primary Topic
- Cancer Genomics and Diagnostics
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Midwestern University