Evaluation of changes in the characteristics of beta2-adrenergic receptors in healthy volunteers under the influence of cholinergics using a modified radioligand analysis technique

Background. Reseaching of the properties of β-adrenergic receptors (β-AR) is an urgent area in both fundamental and applied medicine. One of the most exact ways to determine the characteristics of β-AR is the radioligand method using [125I]cyanopindolol. This method сan determine the binding activity of β-AR on human blood cells. It is especially important to study the receptor link under the influence of various external factors and drugs. Aims — to evaluate the binding activity of β2-AR on T-lymphocytes of peripheral blood cells using radioligand analysis in healthy volunteers and in professional athletes under the influence of cholinergics. Methods. Healthy volunteers and athletes were given methacholine inhalation. Blood sampling was carried out initially on a clean background and after the methacholine test. To level out the differences in the conditions of formulation and to bring the results to the same coordinate system, the evaluation of the characteristics of β-AR in the framework of the study was carried out according to the specific binding index (ISS) — value reflecting the proportion of specific binding of receptors from the total specific. Results. During the study, when dividing volunteers into smokers and non-smokers, a tendency to decrease binding activity was noted. As a result of the study, in the general group of volunteers was registerd a significant decrease in the binding activity of beta2 receptors under the influence of methacholine. In the group of athletes, no significant changes were kept. Conclusions. The reason for these results may be both intense training, which changes the number of receptors, and the presence or absence of eosinophilic inflammation. The smoking factor does not play a significant role.