Development of A Novel EvaGreen-Dye Based Recombinase Aided Amplification Assay Using Self-Avoiding Molecular Recognition System Primers.
The EvaGreen-RAA assay demonstrated significant advantages in terms of simplicity and enhanced sensitivity compared to fluorescent probe-based RAA, offering a novel approach for developing multiplex pathogen detection systems using melting curve analysis.
Authors
- Fengyu Tian
- Zijin Zhao (ORCID: https://orcid.org/0000-0002-7231-2842)
- Xinxin Shen (ORCID: https://orcid.org/0000-0002-4936-9824)
- Lingjun Li (ORCID: https://orcid.org/0000-0003-0056-3869)
- Xuejun Ma (ORCID: https://orcid.org/0000-0001-6733-9621)
- Shaowei Hua
Institutions
- National Institute for Viral Disease Control and Prevention (CN)
Publication Details
- Journal
- PubMed
- Published
- 2025-04-04
- DOI
- https://doi.org/10.46234/ccdcw2025.079
- Citations
- 1
- Primary Topic
- Analytical Chemistry and Sensors
- Type
- article
- Field-Weighted Citation Impact
- 0.74