The Chemical Profiling of Cajanus cajan (L.) Huth (Fabaceae) Seed Oil Extract

This work investigated The Chemical Profiling of Cajanus Cajan (L.) Huth (Fabaceae) Seed Oil Extract using HPLC, GC-MS and FTIR analytical methods. The extraction was done with ethanol to yield a 2.40% yield crude extract. The crude extract was partitioned using hexane, ethyl acetate, dichloromethane and water. The yields of the fractions compared to the 400 g seed used for extraction are 0.30%, 0.46%, 0.36%, 0.65%. The result of the fractions compared to the crude extract (9.6 g) is 17.10%, 25.72%, 20.20% and 36.97 % of the crude extracts, respectively. HPLC analysis revealed the presence of Four (4) prominent phytochemicals: Gallic Acid, Magnoflorine, Rutin and Pinostrobin. The concentration of Gallic Acid, Magnoflorine, Rutin and Pinostrobin in 0.5 mg/mL (500 μg/mL) of cajanus cajan ethanol seed extract was 22.609 μg/mL, 175.236 μg/mL, 169.705 μg/mL and 90.525 μg/mL respectively. The results of the chemical composition of the n- hexane fraction of Cajanus cajan seed oil obtained by GC-MS analysis show nine (9) compounds with a 98% total oil content. The major compounds identified in the seed oil are octadecanoic acid (32.36%), 9, 12-octadecadienoic acid (Z, Z) (29.23%) and linoelaidic acid (25.31%). The FTIR analysis with Aqueous, ethyl acetate and dichloromethane fractions confirms the presence of the following phytochemicals: Esters, flavonoids, Alkaloids, Alcoholic or Phenolic compounds and carbonyl compounds.