A <i>Thermus thermophilus</i> Argonaute (TtAgo) cleavage-aided isothermal amplification strategy (TAC-IAS) for precise detection of locus-specific DNA methylation
A versatile TtAgo cleavage-aided isothermal amplification strategy (TAC-IAS) is devised for precise detection of DNA methylation with high flexibility and universality compared to methylation-restriction endonuclease, reliably identifying methylation with an abundance of 0.1% in a large pool of unmethylated fragments.
Authors
- Bing-Jie Han (ORCID: https://orcid.org/0000-0001-6687-936X)
- Yuanyuan Sun (ORCID: https://orcid.org/0000-0002-2843-739X)
- Chenghui Liu (ORCID: https://orcid.org/0000-0001-8375-0242)
- Hui Tian (ORCID: https://orcid.org/0000-0002-4517-2776)
- Yi Zhao (ORCID: https://orcid.org/0000-0002-3506-9468)
- Xing Chen (ORCID: https://orcid.org/0000-0002-3363-3301)
- Fangfang Sun (ORCID: https://orcid.org/0000-0001-9158-3050)
Institutions
- Zhengzhou University (CN)
- Henan University of Engineering (CN)
- First Affiliated Hospital of Zhengzhou University (CN)
- Shaanxi Normal University (CN)
Publication Details
- Journal
- Chemical Communications
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1039/d5cc02012c
- Citations
- 2
- Primary Topic
- Biological Research and Disease Studies
- Type
- article
- Field-Weighted Citation Impact
- 1.61