Evaluation of the Polymorphic Locus <i>rs1695</i> of the <i>GSTP1</i> Gene Association with HPV Infection in Women in Burkina Faso

Introduction: Genetic polymorphisms of certain classes of glutathione S-transferase (GST), the enzyme responsible for the biotransformation of drugs and xenobiotics, have been associated with the risk of several cancers such as cervical cancer. The objective of this study was to characterize the impact of the rs1695 polymorphism of GSTP1 in women infected by high-risk human papillomavirus (HR-HPV) in Ouagadougou, Burkina Faso. Methods: Genotyping of GSTP1 rs1695 polymorphisms was performed in 55 women with high-risk HPV infection, and 89 healthy controls using the PCR-RFLP method. Conventional PCR was used for DNA amplification and the enzymes Alw26I or BsmA1 were used for enzymatic digestion. Results: The prevalence of GSTP1 rs1695 AA, AG and GG genotypes was respectively 27.8%, 45.8% and 26.4% in the study population with a mutation rate of 49.31%. However, the frequency of AA, AG and GG genotypic was respectively 30.3%, 45%, 24.7% in controls and 23.6%, 47.3%, 29.1% in cases. Conclusion: Our study allowed us to characterize the frequencies of GSTP1 rs1695 genotypes in the study population, in cases and controls. From our analysis, none of the three genotypes of GSTP1 rs1695 weren’t associated with a risk or a protective factor for HR-HPV infection in women in Burkina Faso.