Use of microsatellite markers to characterize of dogs Cavalier King Charles spaniel breed of different breeding

For genetic characterization of dogs of Cavalier King Charles Spaniel breed, a comparative analysis of genotypes by STR loci of DNA of adult dogs was performed. The animals were divided into four groups distinguishable by color. From 1 to 5 allelic variants per locus were revealed with an average number of alleles of 2.9 per locus. Three alleles were found in the loci AHTk211 1, CXX279 1, REN169O18 1, INU005 1, AHTh171 1. Four alleles were found in the loci AHTh260 1, REN54P11 1, AHT137 1, AHTk253 1, REN162C04 1. The locus REN169D01 was characterized by the maximum number of alleles (5 alleles) with the number of effective alleles of 3.0. The fi xation index F (Fisher-Ryder criterion) in different groups of dogs of Cavalier King Charles Spaniel breed varied from –0.636 to –0.091. The negative deviation of the fixation index indicated an excess of heterozygotes identified in the four studied groups of dogs. Analysis of the genetic identity indices according to Ney showed that the level of genetic similarity between the groups of dogs ranged from 0.793 to 0.902. For example, the 2nd group was identical to the 3rd group (0.857), and the 1st and 4th groups were identical (0.793). Comparative analysis showed that the 1st and 2nd groups of dogs had a high degree of genetic identity between themselves (0.828), 2nd and 4th (0.829), 3rd and 4th (0.821), while the 1st and 3rd, 1st and 4th groups were more distant according to Nei. Thus, there is a different genetic identity between the studied groups of dogs by color, which must be taken into account in further breeding work with the breed Cavalier King Charles Spaniel.