Determination of sulphamethoxazole and carbamazepine in waste and surface water of Shymkent city

Aim . The development of a liquid chromatography method for the quantitative determination of Sulfamethoxazole and Carbamazepine in wastewater from sewage systems and surface waters of the city of Shymkent. Materials and methods . The chromatographic system DIONEX UltiMate 3000 with diode-array detector at absorption wavelength of 254 nm, reversed-phase version with mobile phase of acetonitrile-water composition (40: 60) and with a Hypersil GOLD C8 150x2.1 mm 1.9 micron column filled with porous ultrapurified silica gel, the thermostat temperature of the chromatographic column was 30 0C. Elution was carried out in isocratic mode. The total analysis time for 1 sample was 30 min. The flow rate of mobile phase was 1 ml/min. The retention time of standard sample solutions of carbamazepine was 3.612±0.1 min, for sulfamethoxazole – 6.910±0.1 min. Results and discussion . A validated method for the detection and quantification of drug residues in water samples using the UHPLC-DAD method has been developed: the correlation coefficient of the linear regression graph was 0.9999; the relative standard deviation of the method for sulfamethoxazole and carbamazepine between samples within a cycle was 0.08110.7354%, between cycles – 0.1660-1.6457%. Monitoring of drug contamination of the studied waste and surface waters were carried out. At low concentrations, sulfamethoxazole and carbamazepine were detected and quantified in waste and surface waters of the Shymkent city. Under chromatographic conditions, the retention time of carbamazepine was 3.612±0.1 min, of sulfamethoxazole – 6.910±0.1 min, which corresponds to the retention times of the standard sample solutions. Conclusions . A method for the study of water samples for the content of pharmaceutical residues using HPLC-DAD was developed and validated: the correlation coefficient of the linear regression graph was 0.9999; the relative error for carbamazepine ranged from 0.0166% to 1.6457%, and for sulfamethoxazole from 0.3888% to 0.8212%, confirming the high reproducibility of the developed method, which is suitable for further analytical research. Based on the results of the study of water samples, the quantitative content of carbamazepine and sulfamethoxazole in wastewater and surface waters of Shymkent was determined during the initial study in the autumn period. The results of preliminary studies form the basis for further research on wastewater and surface waters for the presence of pharmaceutical residues to monitor the ecological situation in the region.