The knowledge driven DBTL cycle provides mechanistic insights while optimising dopamine production in Escherichia coli
In essence, a highly efficient dopamine production strain was developed by implementing the knowledge driven DBTL cycle involving upstream in vitro investigation. The fine-tuning of the dopamine pathway by high-throughput RBS engineering clearly demonstrated the impact of GC content in the Shine-Dalgarno sequence on the RBS strength.
Authors
- Ralf Takors (ORCID: https://orcid.org/0000-0001-5837-6906)
- Natalia Viktorovna Trachtmann (ORCID: https://orcid.org/0000-0003-3291-8329)
- Lorena Hägele (ORCID: https://orcid.org/0009-0001-6624-3457)
Institutions
- University of Stuttgart (DE)
- Kazan Scientific Center (RU)
- Kazan Institute of Biochemistry and Biophysics (RU)
- Institute of Mechanics and Engineering (RU)
Publication Details
- Journal
- Microbial Cell Factories
- Published
- 2025-05-16
- DOI
- https://doi.org/10.1186/s12934-025-02729-6
- Citations
- 2
- Primary Topic
- Gene Regulatory Network Analysis
- Type
- article
- Field-Weighted Citation Impact
- 1.61
Funders
- Universität Hohenheim
- Deutsche Forschungsgemeinschaft
- Universität Stuttgart