Identification of Dengue Virus through Sero-Epidemiological Methods in Individuals of District Tank, Khyber Pakhtunkhwa

This study aimed to evaluate the serological characteristics of patients infected with dengue in 2024 at District Tank, Khyber Pakhtunkhwa, Pakistan. In 2024, a total of 100 individuals visited the hospital. Blood specimens from patients in the pathology laboratory. The data on dengue infection was obtained from the District Health Office in Tank. In the designated dengue patient ward, a patient with dengue fever who was showing little symptoms was admitted. For a period of five months, beginning in August and ending in December, the data was collected from hospitals. It was necessary to utilize a distinct form to consider the gender and history of the users. Furthermore, the Immunochromatographic Technique (ICT) was used in the laboratory screening of a patient suspected of having dengue fever. The collected blood samples underwent screening for Dengue infection utilizing the BIOLINE Dengue NS 1 Ag+Ab Combo Test. A total of 100 blood samples underwent screening. Out of the total samples, 28 tested positives for dengue fever, representing a percentage of 28%, while 72 samples, accounting for 72%, were found to be negative. Regarding patient age groups, 22 patients were found positive for Dengue infection, aged between 15 and 35 years, representing 78.57%, which constitutes most Dengue-infected patients. A significant epidemic of Dengue severity among patients was seen in September and October, attributed to the wet and post-rainy season. It is determined that biological, environmental, pharmacological, and educational strategies should be employed to combat illness. In addition, there is a requirement for the formation of teams to investigate the true origin of dengue disease and its potential effects on the residents of the region.