Pilot study on the effectiveness of Sidhakarya meditation in reducing malondialdehyde levels: an experimental approach

Introduction: Oxidative stress, caused by an imbalance between reactive oxygen species (ROS) and antioxidants, contributes to chronic diseases. Malondialdehyde (MDA), a marker of oxidative damage, is commonly used to assess this stress. Meditation, especially mindfulness practices, has shown potential in reducing oxidative stress. This pilot study investigates the impact of Sidhakarya Meditation, a specific mindfulness-based technique, on MDA levels in individuals experiencing moderate stress. Objective: To evaluate the effectiveness of Sidhakarya Meditation in reducing MDA levels, a key biomarker of oxidative stress. Methodology: A randomized pre-test and post-test control group design was employed. A total of 60 participants (mean age 34.5 ± 8.2 years) were randomly assigned to the intervention group (Group B), which practiced daily 30-minute Sidhakarya meditation sessions for 6 weeks, or the control group (Group A), which maintained their usual activities. MDA levels were measured using the thiobarbituric acid reactive substances (TBARS) assay. Psychological stress and mood were assessed using the Perceived Stress Scale (PSS) and the Profile of Mood States (POMS). Results: Of the 60 participants, 55 completed the study. The intervention group showed a 30% reduction in MDA levels (from 1780 nmol/mL to 1242 nmol/mL, p = 0.01), along with significant improvements in stress (p = 0.04) and mood (p = 0.03). Discussion: Sidhakarya Meditation effectively reduced oxidative stress and improved emotional well-being, supporting its potential as a non-pharmacological intervention for stress management. Conclusions: This study highlights Sidhakarya Meditation as a promising approach for reducing oxidative stress and improving psychological health.