Isolation of Human Metapneumovirus from clinical specimen in human organoid-derived bronchial cell cultures is superior to isolation in monolayer cell line cultures
Overall, the isolation efficiency of serotype A HMPV was comparable in both models, while isolation of serotype B viruses was profoundly more efficient in the ODB cultures than in Vero-118 cells, suggesting that primary cultures expressing ciliated cells should be considered as a superior isolation method for HMPV from clinical specimens.
Authors
- Jeroen J. A. van Kampen (ORCID: https://orcid.org/0000-0002-7625-8230)
- Ron A. M. Fouchier (ORCID: https://orcid.org/0000-0001-8095-2869)
- Bernadette van den Hoogen (ORCID: https://orcid.org/0000-0003-1996-8559)
- Stefan van Nieuwkoop (ORCID: https://orcid.org/0000-0002-4694-6833)
- Babs E. Verstrepen (ORCID: https://orcid.org/0000-0002-4666-0798)
- Mathis Funk (ORCID: https://orcid.org/0000-0001-7830-9989)
- Pau Ribó-Molina (ORCID: https://orcid.org/0009-0004-9646-0593)
Institutions
- Erasmus MC (NL)
- Erasmus University Rotterdam (NL)
Publication Details
- Journal
- Journal of Clinical Virology
- Published
- 2025-05-15
- DOI
- https://doi.org/10.1016/j.jcv.2025.105805
- Primary Topic
- Respiratory viral infections research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Horizon 2020 Framework Programme
- H2020 Marie Skłodowska-Curie Actions
- HORIZON EUROPE Marie Sklodowska-Curie Actions