Effect of Centrifugation Speed and Blood Tubes with Different Contents on Certain Serum/Plasma Biochemical Parameters in Cocks

This study was carried out to investigate the effect of blood tubes with different anticoagulants and different spinning speed applications in the blood centrifuge on biochemical analyses. The study conducted with a total of 16 Gerze cocks, a native breed of Türkiye. In the first part of the study, 8 roosters were slaughtered and the blood taken was transferred to lithium heparinized tubes only. Then, the blood was centrifuged at 3000, 4000 and 5000 rpm for 10 minutes. Blood samples were taken into plain-tubes, K3EDTA and lithium heparin tubes, and centrifuged at 3000 rpm for 10 minutes. The study results indicated that, centrifugation speed had no statistically significant effect on the levels of plasma glucose (p = 0,691), triglyceride (p = 0,451), total cholesterol (p = 0,993), high-density lipoprotein [(HDL-cholesterol) (p = 0,917)], low-density lipoprotein [(LDL-cholesterol) (p = 0,990)], total protein (p = 0,860), inorganic phosphorus (p = 0,885), calcium (p = 0,980), amount of plasma (p = 0,903) and, aspartate aminotransferase activities [(AST) (p = 0,692)]. In the second part of the study 8 different cocks apart from first part of the study, were slaughtered and the blood taken into tubes with different contents as plain tubes (serum), lithium heparinized tubes (plasma) and K3EDTA tubes (plasma). Same parameters with first part of the study were investigated. The results showed that no statistical difference found between serum or plasma obtained from blood tubes with different contents (p˃0.05). Additionally, the calcium levels could not be analyzed with K3EDTA plasma. Consequently, we observed that centrifugation speeds up to 5000 rpm did not affect the parameters frequently investigated in scientific research and routine analysis in poultry Similarly, in the tube content experiment, no statistical difference was found in other parameters.