Evaluation of Microbial Growth in Dried Blood Stains

Blood is one of the most critical type of biological fluid recovered from crime scene and often crucial in reconstructing events, identifying individuals and time since deposition. This study focuses on the microbial aspect of the blood as blood provide an ideal environment for microbial growth due to its nutrient content. Microbial growth in dried bloodstains occurs in distinct phases and is influenced by environmental conditions such as temperature, humidity and exposure to air. The suggested approach is experimental, requires blood samples that are deposited on any surface and collected at various time intervals (6, 24, 48, 72, 96 hours). The study aims to investigate microbial growth dynamics in bloodstains over time and their impact on the spectral properties measured through UV-Vis spectrophotometer. The turbidometeric analysis measured the absorbance of rehydrated sample at 600nm to quantify microbial proliferation. This approach bridges forensic science and microbiology, ensuring a foundation for further researches in future.