Comparison of MALDI-TOF MS instruments and databases for the identification of uncommon yeasts, <i>Aspergillus</i> spp. and rare filamentous fungi
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-ToF-MS) is key for fungal identification nowadays. We present here the largest comparison of MALDI-ToF-MS instruments and databases focusing on rare yeasts and molds identification, challenging the existing instruments and both commercial and academic databases. The strains were collected in Saint Louis Hospital (Paris) and identified using bar-code sequencing. We showed that commercial databases are efficient for the identification of the main fungal species tested, whereas the academic database outperforms them for the identification of cryptic species.
Authors
- Marc Lemaire (ORCID: https://orcid.org/0000-0003-2800-0590)
- Renaud Piarroux (ORCID: https://orcid.org/0000-0002-4151-4134)
- J. Bui
- Anne‐Cécile Normand (ORCID: https://orcid.org/0000-0002-9589-4603)
- Sarah Dellière (ORCID: https://orcid.org/0000-0003-3821-1762)
- Théo Ghelfenstein-Ferreira (ORCID: https://orcid.org/0000-0002-7784-4434)
- Alexandre Alanio (ORCID: https://orcid.org/0000-0001-9726-3082)
- Élodie Da Silva (ORCID: https://orcid.org/0000-0001-8289-1262)
- Véronique Charlier
- Samia Hamane
- Marion Dutkiewicz
- Maéva Garros
Institutions
- Institut Pasteur (FR)
- Université Paris Cité (FR)
- Sorbonne Université (FR)
- Pitié-Salpêtrière Hospital (FR)
- Hôpital Saint-Louis (FR)
Publication Details
- Journal
- Journal of Clinical Microbiology
- Published
- 2025-05-15
- DOI
- https://doi.org/10.1128/jcm.01612-24
- Citations
- 3
- Primary Topic
- Bacterial Identification and Susceptibility Testing
- Type
- article
- Field-Weighted Citation Impact
- 3.96