A novel mutation of <i>ramR</i> involved in tigecycline resistance in <i>Klebsiella pneumoniae</i>
In this study, a novel missense mutation (g. 517_518 del GC) was detected in the <i>ramR</i> of tigecycline-induced <i>Klebsiella pneumoniae</i>, which was conducted <i>in vitro</i>, and the effects of anti-tigecycline caused by this mutation in <i>ramR</i> were confirmed. A high expression of <i>ramR</i> was observed in all tigecycline-induced strains. In addition, g. 517_518 del GC in <i>ramR</i> maintained tigecycline resistance. In summary, we illustrated a novel mutation of <i>ramR</i>, leading to tigecycline resistance in <i>Klebsiella pneumoniae</i>.
Authors
- Feinan Qian (ORCID: https://orcid.org/0000-0002-4316-115X)
- Hong Du (ORCID: https://orcid.org/0000-0001-6652-4702)
- Haifang Zhang (ORCID: https://orcid.org/0009-0007-9146-1265)
- Yuyao Xu (ORCID: https://orcid.org/0000-0003-1097-483X)
- Qi Chen (ORCID: https://orcid.org/0000-0001-6353-9589)
- Lei Wang (ORCID: https://orcid.org/0000-0002-6180-9802)
- Chenhao Zhao (ORCID: https://orcid.org/0009-0005-8252-9235)
- Xin Ma (ORCID: https://orcid.org/0009-0002-7131-4245)
- Xiangyu Guan (ORCID: https://orcid.org/0000-0001-9396-8245)
Institutions
- Soochow University (TW)
- Soochow University (CN)
- Second Affiliated Hospital of Soochow University (CN)
- CM Hospital (KR)
- Yangzhou University (CN)
Publication Details
- Journal
- Microbiology Spectrum
- Published
- 2025-05-15
- DOI
- https://doi.org/10.1128/spectrum.03204-24
- Primary Topic
- Antibiotic Resistance in Bacteria
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China