Transcriptomic profiling of cytomegalovirus infection in cardiac transplantation: proof-of-concept for a new strategy in tissue markers application
We have identified a distinctive combined molecular profile of mRNAs and miRNAs for infection in post-cardiac transplant follow-up. Based on IL7R, GZMK, mir-93-5p, and mir-345-5p we suggest a novel possible workflow to distinguish infection, where those markers are downregulated, from rejection, where they are overexpressed, on EMB specimens. This analysis showed good accuracy and promising predictive performance. The future combined analysis of these genes and these miRNAs through user-friendly techniques, such as quantitative PCR, could reduce turn-around time and improve our diagnostic power for distinguishing CMV infection from ACR in EMB specimens.
Authors
- Giuseppe Toscano (ORCID: https://orcid.org/0000-0001-6827-5850)
- Francesco Tona (ORCID: https://orcid.org/0000-0003-4828-7875)
- Ilaria Barison (ORCID: https://orcid.org/0000-0001-6745-1605)
- Chiara Tessari (ORCID: https://orcid.org/0000-0001-9011-1625)
- Marny Fedrigo (ORCID: https://orcid.org/0000-0002-3081-2937)
- Luca Vedovelli (ORCID: https://orcid.org/0000-0003-4847-2333)
- Alessia Giarraputo (ORCID: https://orcid.org/0000-0003-4070-7943)
- Susanna Mandruzzato (ORCID: https://orcid.org/0000-0002-0707-995X)
- Elisabetta Rossi (ORCID: https://orcid.org/0000-0002-5242-9568)
- Darío Gregori (ORCID: https://orcid.org/0000-0001-7906-0580)
- Annalisa Angelini (ORCID: https://orcid.org/0000-0002-3578-4488)
- Gino Gerosa (ORCID: https://orcid.org/0000-0002-6261-699X)
- Cristina Basso (ORCID: https://orcid.org/0000-0002-0195-9753)
- Chiara Castellani (ORCID: https://orcid.org/0000-0002-0275-4864)
- Sonia Minuzzo (ORCID: https://orcid.org/0000-0003-2588-6058)
- Nicola Pradegan (ORCID: https://orcid.org/0000-0002-5129-5190)
- Davide Abate (ORCID: https://orcid.org/0000-0002-2387-729X)
- Diego Perazzolo
Institutions
- University of Padua (IT)
- Istituto Oncologico Veneto (IT)
Publication Details
- Journal
- Frontiers in Immunology
- Published
- 2025-05-16
- DOI
- https://doi.org/10.3389/fimmu.2025.1581151
- Primary Topic
- Cytomegalovirus and herpesvirus research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Università degli Studi di Padova