Convergence and divergence of B cell responses in two HIV-1 Env immunizations in Rhesus macaques
Multiclade and sequential regimens before SOSIP-stabilized Env immunization elicited B-cells that converge on shared epitopes. A change in immunogen format results in a divergent B-cell response that vastly fails to engage prior responses. Critically, B-cell priming with non-stabilized Env cannot modify the effect of the epitope immunodominance hierarchy in a SOSIP trimer. These results suggest that a change in immunogen format may cause off-target B-cell engagement, but also that B-cell repriming is possible despite pre-existing immunity.
Authors
- Justin Pollara (ORCID: https://orcid.org/0000-0003-1892-4722)
- M. Anthony Moody
- Guido Silvestri (ORCID: https://orcid.org/0000-0001-6602-2470)
- Shuqin Gu
- David B. Weiner (ORCID: https://orcid.org/0000-0002-2232-8512)
- Donatella Negri (ORCID: https://orcid.org/0000-0002-3437-9288)
- Shalini Jha (ORCID: https://orcid.org/0000-0002-9792-2161)
- Melissa Kerkau (ORCID: https://orcid.org/0000-0002-4388-8498)
- Jenna M DeLuca (ORCID: https://orcid.org/0000-0001-6390-8436)
- Diane G. Carnathan (ORCID: https://orcid.org/0009-0001-7073-3723)
- Mansi Purwar
- Guido Ferrari (ORCID: https://orcid.org/0000-0001-7747-3349)
- Xiaoying Shen (ORCID: https://orcid.org/0000-0002-8387-3952)
- Mattia Bonsignori (ORCID: https://orcid.org/0000-0003-2973-2101)
- Andrea Cara (ORCID: https://orcid.org/0000-0003-4967-1895)
- Kevin J. Wiehe (ORCID: https://orcid.org/0000-0001-9933-9618)
- Maria Blasi (ORCID: https://orcid.org/0000-0002-7091-0312)
- Kurt Wollenberg (ORCID: https://orcid.org/0000-0002-4973-9370)
- Kevin O. Saunders (ORCID: https://orcid.org/0000-0001-7399-7954)
- Shan Lu
- Taylor J. McGee (ORCID: https://orcid.org/0000-0001-6273-1915)
- Mary E. Klotman (ORCID: https://orcid.org/0000-0002-7084-0406)
Institutions
- The Wistar Institute (US)
- Emory University (US)
- University of Massachusetts Chan Medical School (US)
- Duke University (US)
- Istituto Superiore di Sanità (IT)
- National Institute of Allergy and Infectious Diseases (US)
Publication Details
- Journal
- Communications Medicine
- Published
- 2025-05-15
- DOI
- https://doi.org/10.1038/s43856-025-00899-3
- Primary Topic
- HIV Research and Treatment
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Division of Intramural Research, National Institute of Allergy and Infectious Diseases
- National Institutes of Health
- National Institute of Allergy and Infectious Diseases
- NHLBI Division of Intramural Research