Biological sample desalting and buffer replacement based on electromigration in a discontinuous electrolyte system using a 3D printed device
The device enables fast and effective purification of highly complicated samples. It can be easily scaled for the available sample amount (ranging from microliters to milliliters) and the analyte size of interest by selecting appropriate membrane pore sizes. The device's applicability was optimized on DNA samples with high salt content and tested on plasma samples. But it also has the potential for purification/desalting of a wide range of biological compounds, including proteins, peptides, or charged oligosaccharides, as well as organelles, cells, viruses, or bacteria.
Authors
- František Foret (ORCID: https://orcid.org/0000-0002-0060-7350)
- Yann Astier (ORCID: https://orcid.org/0000-0001-5036-971X)
- Doo Soo Chung (ORCID: https://orcid.org/0000-0002-8335-3201)
- Ivona Voráčová (ORCID: https://orcid.org/0000-0002-2885-5583)
- Vanda Kocianová (ORCID: https://orcid.org/0009-0006-9845-4175)
Institutions
- Seoul National University (KR)
- Masaryk University (CZ)
- Bexley Hall (US)
- Czech Academy of Sciences, Institute of Analytical Chemistry (CZ)
Publication Details
- Journal
- Analytica Chimica Acta
- Published
- 2025-05-15
- DOI
- https://doi.org/10.1016/j.aca.2025.344198
- Primary Topic
- 3D Printing in Biomedical Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- European Commission
- Ministerstvo Školství, Mládeže a Tělovýchovy
- Grantová Agentura České Republiky
- National Research Foundation of Korea
- Akademie Věd České Republiky
- Ústav analytické chemie, Akademie Věd České Republiky