Enhanced nattokinase production by Bacillus subtilis from glycerol and okara: optimization of culture medium via response surface methodology
The genetic modifications enabling efficient glycerol metabolism in Bacillus subtilis 13,932, along with the optimized fermentation strategy using renewable resources, significantly improved nattokinase production. The high enzymatic activity achieved demonstrates the potential of this approach for cost-effective and sustainable nattokinase production.
Authors
- Yongle Duan (ORCID: https://orcid.org/0000-0002-8164-9818)
- Ning Hao (ORCID: https://orcid.org/0000-0003-0920-6374)
- Wenjun Du
- Gege Guo
- Zhaoxing Liu
- Xu Yang (ORCID: https://orcid.org/0000-0002-2006-9406)
Institutions
- The Synergetic Innovation Center for Advanced Materials (CN)
Publication Details
- Journal
- Frontiers in Microbiology
- Published
- 2025-05-14
- DOI
- https://doi.org/10.3389/fmicb.2025.1577292
- Citations
- 5
- Primary Topic
- Enzyme Production and Characterization
- Type
- article
- Field-Weighted Citation Impact
- 6.16
Funders
- National Key Research and Development Program of China