Mining Biosynthetic Gene Clusters of Bacillus subtilis MGE 2012 Using Whole Genome Sequencing

This study aims to elucidate the genomic characteristics of Bacillus subtilis MGE 2012, a strain isolated from Korean traditional fermented food, meju, which contributes to its high enzyme activity and potential applications. The whole genome sequence of B. subtilis MGE 2012 was assembled using MEGAHIT, annotated using RAST and BLASTKOALA v3.1. Phylogenetic analysis placed MGE 2012 within the Bacillus clade, showing high similarity to B. subtilis NCIB 3610 and B. subtilis ATCC 6051. AntiSMASH analysis identified 14 biosynthetic gene clusters (BGCs) capable of producing various secondary metabolites, including subtilosin, bacillibactin, fengycin, bacilysin, plipastatin, and surfactin. This study provides an overview of the whole genome and secondary metabolite profile of B. subtilis MGE 2012, emphasizing its potential applications in biotechnology. While the primary focus of this study was to explore the genomic characteristics and secondary metabolite profile, future research could delve deeper into genome mining for enzyme activities and their applications.