Biotransformation of tobacco-derived Z-abienol into precursors of ambrox by newly identified Acinetobacter tjernbergiae LSC-2

Z-abienol is a labdane diterpene present in tobacco leaves and is a key precursor for producing valuable aroma compounds such as ambrox. This study aimed to identify and characterize a bacterial strain that can efficiently degrade Z-abienol through microbial fermentation. The strain LSC-2 isolated from fresh tobacco leaves was identified as <i>Acinetobacter tjernbergiae</i> based on its morphological features and 16S rDNA phylogenetic analysis. Results of fermentation optimization experiments showed that the highest degradation efficiency of LSC-2 (69.3%) was achieved under the following conditions: 1 mg/mL Z-abienol, 0.5 mg/mL urea as the nitrogen source, pH 7, 30°C, and 150 rpm over 4 days. Whole-genome sequencing and functional annotation revealed that oxidoreductases, especially those from the auxiliary activity enzyme family, play a critical role in Z-abienol degradation. High-performance liquid chromatography and gas chromatography-mass spectrometry analysis confirmed the biotransformation of Z-abienol into various intermediates, including sclareol (211.3 μg/mL), scalaral (89.5 μg/mL), and amberonne (57.0 μg/mL). These intermediates have significant industrial applications, particularly in fragrance, pharmaceutical, and cosmetic industries. Sclareol serves as a key precursor in ambrox synthesis, a widely used fixative in high-end perfumery, whereas scalaral and amberonne enhance the aroma in tobacco and flavor formulations. The findings of this study provide valuable insights into the microbial degradation of Z-abienol, which will help develop a sustainable approach to producing bio-based fragrance compounds. Future studies should focus on enzymatic mechanisms and metabolic engineering strategies to improve the efficiency of biotransformation.