THE MAIN METHODOLOGICAL PROBLEMS OF MULTI-CRITERIA ANALYSIS OF THE EFFECTIVENESS OF PARASITOLOGICAL EXAMINATION

he present scientific research is aimed at systematizing general approaches to the choice of multiplex PCR in clinical and sanitary parasitology. Our data are based on the need to focus the laboratory service, which carries out parasitological control of environmental safety, on the development and organization of multicomplex studies, which, based on a provide the most comprehensive data for a factor analysis and multi-criteria assessment of the parasitological situation in the country. The purpose of the study: Conducting an analysis of current trends and our own multicriteria data for evaluating the effectiveness of laboratory tests based on PCR analysis in parasitology. Materials and methods. The analysis of modern literature data from peer-reviewed sources (PubMed) has been carried out to substantiate the scientific purpose of the study. A series of experimental studies of two sets of multiplex PCR (qPCR) tests for the detection of 7 helminth species contained in 192 model samples was carried out. Research results. The methodological limitations and advantages of using microscopic and multimplex PCR studies are determined. The limits of detection of DNA from helminth eggs and fragments of imago tissues of round and flat helminths stored for a long time in preservative, as well as in freshly isolated biomaterial, have been established at the level of 101-103 in 100 µl and the diagnostic sensitivity of PCR tests at the level of 70,0- 90,0 %. Conclusion. It is proposed to develop an innovative form of multi-criteria assessment of environmental parasitic safety based on the widespread use of multiplex PCR (qPCR) tests, and in the future, with the introduction of digital PCR (dPCR) to simultaneously determine the qualitative and quantitative characteristics of pathogenic contaminants of different types when testing uniform samples. Based on the data obtained, the necessity of active development of PCR technologies and their wide application in parasitology in the laboratory service of state control bodies is substantiated.