Protocol for determining regulators of competence in regeneration of adventitious roots using hypocotyl wounding approach in Arabidopsis

Plant plasticity enables the regeneration of adventitious roots upon wounding. Here, we present a protocol for inducing adventitious roots in wounded Arabidopsis hypocotyls, phenotyping, and spatiotemporal gene expression analysis. We also describe the steps for quantifying early adventitious root developmental stages. This protocol can be used to assay genetic backgrounds and chemical treatments to understand their role in regulating competency for regeneration upon wounding of the hypocotyl. For complete details on the use and execution of this protocol, please refer to Damodaran et al.¹.