Gelofusine as alternative to Dextran40‐based solution for washing cryopreserved hematopoietic stem cell products prior to infusion: Validation and application to clinical practice
For 10 CBUs washed with Gelofusine, CD34<sup>+</sup> and TNC viability and recovery were 96%, 87%, 71%, and 75% respectively, higher than our reference values. In transplanted products, CD34<sup>+</sup> and TNC viability and recovery were 96%, 89%, 82%, and 91% respectively. Comparisons with Rubinstein solution revealed similar TNC and CD34<sup>+</sup> recovery but significantly higher TNC (89% vs. 68%) and CD34<sup>+</sup> (97% vs. 89%) viability with Gelofusine. Engraftment times for both solutions were similar. These findings support Gelofusine as an effective and valid alternative to Rubinstein-solution for washing cryopreserved HSCs.
Authors
- Peppino Mirabelli (ORCID: https://orcid.org/0000-0002-2183-7577)
- Laura Auriemma
- Maria Esposito (ORCID: https://orcid.org/0000-0002-4773-6993)
- Giovanna Maisto
- Alessandra Picardi (ORCID: https://orcid.org/0000-0002-0136-7686)
- Fabiana Cacace (ORCID: https://orcid.org/0009-0006-8788-8461)
- Roberta Penta (ORCID: https://orcid.org/0000-0001-6441-0525)
- Donato Madalese (ORCID: https://orcid.org/0000-0002-9971-7313)
- Stefania Nappo (ORCID: https://orcid.org/0000-0002-7536-7806)
- Mario Toriello
- Francesco Paolo Tambaro (ORCID: https://orcid.org/0000-0002-9145-3691)
- Francesco Topo
- Lucia Gallo
- Giovanni Castellano
Institutions
- Ospedale Antonio Cardarelli (IT)
Publication Details
- Journal
- Transfusion
- Published
- 2025-05-14
- DOI
- https://doi.org/10.1111/trf.18272
- Primary Topic
- Hematopoietic Stem Cell Transplantation
- Type
- article
- Field-Weighted Citation Impact
- 0.00