Establishment of Reference Measurement Procedure for TP53 R175H/R248W Detection and a Novel Preparation Method for ctDNA Reference Material
Firstly, accurate measurement procedures for TP53-R175H and TP53-R248W variants based on dPCR were established in this study. Furthermore, a protocol for preparing ctDNA reference material was established here. By digesting nucleosomal DNA derived from cancer cell lines with micrococcal nuclease, this method can closely mimic the properties of clinical ctDNA. The dPCR method and ctDNA reference material preparation approach established here could be used in ctDNA detection and for improving its reliability.
Authors
- Jingya Yang (ORCID: https://orcid.org/0000-0002-7419-5133)
- Chunyan Niu (ORCID: https://orcid.org/0000-0003-2330-937X)
- Lianhua Dong (ORCID: https://orcid.org/0000-0002-8609-9552)
- Jiejie Zhang (ORCID: https://orcid.org/0009-0006-1419-0636)
- Yanru Tang
Institutions
- National Institute of Metrology (CN)
- Shanghai Ocean University (CN)
Publication Details
- Journal
- Genes
- Published
- 2025-05-14
- DOI
- https://doi.org/10.3390/genes16050576
- Primary Topic
- Molecular Biology Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Institute of Metrology, China