Alleviating the Effects of Electrolyzed Alkaline Water on Hyperuricemia in Mice

<b>Objectives:</b> This study investigated the effects and mechanisms of electrolyzed alkaline water (EAW), a type of drinking water, on hyperuricemia (HUA) in mice. <b>Methods:</b> A hyperuricemia model was established by intraperitoneal injection of potassium oxonate and free access to a high-purine diet. EAW was provided ad libitum for 21 days. <b>Results:</b> The results showed that EAW had little impact on the levels of blood urea nitrogen, alanine aminotransferase, aspartate aminotransferase, albumin, or xanthine oxidase in mice (<i>p</i> > 0.05). Interestingly, EAW ingestion induced significant reductions in uric acid and creatinine levels (<i>p</i> < 0.05), along with increased urinary uric acid excretion (<i>p</i> < 0.05) and less renal pathological changes in mice. Additionally, EAW upregulated GLUT9 gene expression (<i>p</i> > 0.05) and downregulated URAT1 protein expression. <b>Conclusions:</b> In conclusion, this study demonstrates that EAW promotes uric acid excretion by downregulating URAT1 and GLUT9 protein expression, resulting in a significant reduction in uric acid levels.