Optimization of sustainable bioprocessing for the production of high-value biomolecules (chlorophylls and proteins) using <i>Desmodesmus subspicatus</i>

Traditional protein and pigment (e.g., chlorophyll) sources are becoming insufficient due to the rapid rise of the global population in modern civilization. Microalgae offer a promising solution for protein and chlorophyll sources due to their higher productivity than terrestrial plants. This study aims to optimize the cultivation conditions for <i>Desmodesmus subspicatus</i>, a microalgal strain containing ∼60% protein and 4% chlorophyll, to enhance biomass, protein and chlorophyll productivity. A Taguchi Orthogonal Array (TOA) was used for systematic optimization of BG-11 medium components. Further experiments assessed the effects of light intensity and different carbon and nitrogen sources. Under optimized BG-11 conditions, biomass increased 1.3-fold, with protein and chlorophyll productivity rising 2.25 and 1.92-fold, respectively. Supplementation with carbon and nitrogen sources under varying light (84-504 µmol m^-2 s^-1) further enhanced yields by 1.6-fold. Glycine proved to be the most effective nitrogen source, while cellulose as a carbon source resulted in 2.4-fold higher biomass, 7.3-fold higher protein, and 2.3-fold higher chlorophyll. Cytotoxicity assessment of the extracted chlorophyll revealed over 94% A549 cell viability at concentrations up to 100 µg/mL, confirming its biocompatibility. Therefore, <i>Desmodesmus subspicatus</i> has promise as a sustainable source of proteins and chlorophylls in the nutraceutical and food industries.