Leuconostoc mesenteroides M13: optimization of bioprocesses and antioxidant property for food industry application
Several reactive oxygen species are related to physiological functions but also play roles in the development of certain human diseases. Reactive oxygen species are counterbalanced by a physiological defense network such as antioxidants. Probiotic strains have been used as natural antioxidants, but its viable scalability is a challenger. The objective of this work was to optimize the bioprocesses of Leuconostoc mesenteroides M13 and evaluate its antioxidant activity and its shelf-life. The optimized production of M13 biomass was 2,542% greater than non-optimized processes, resulting in a yield of 2.67 × 1010 CFU mL-1 under conditions comprising 13.67 g L-1 of yeast extract and .73 g L-1 of magnesium sulfate. The aggregated spherical structure observed in scanning electron microscopy may have a certain protective effect. The viability of the lyophilized strain storage at 4°C was maintained for a period of up to 8 months. Antioxidant activity assays revealed efficacies for intact and lysed cells as 81 and 65%, respectively. The survival rate of the M13 strain in the presence of hydrogen peroxide was observed at 86% (1.0 mM for 8 h). M13 demonstrated a cost-effective strategy for probiotic application in the food industry as an antioxidant supplement.
Authors
- José Mauro Granjeiro (ORCID: https://orcid.org/0000-0002-8027-8293)
- Daniela Regiane da Silva (ORCID: https://orcid.org/0009-0005-8781-2830)
- Vinícius Souza Tarabal (ORCID: https://orcid.org/0000-0002-8736-8531)
- Daniel Bonoto Gonçalves (ORCID: https://orcid.org/0000-0002-8178-1026)
- Adriano Guimarães Parreira (ORCID: https://orcid.org/0000-0002-7770-7165)
- Heloísa Carneiro COLARES (ORCID: https://orcid.org/0000-0002-3354-5774)
- Celso Barbosa de SANT’ANNA FILHO (ORCID: https://orcid.org/0000-0001-9759-9512)
- Hiure Gomes Ramos Meira (ORCID: https://orcid.org/0000-0001-8563-3170)
- Juliana Teixeira de Magalhães (ORCID: https://orcid.org/0000-0002-0532-7323)
- Vanessa Faria Cortes (ORCID: https://orcid.org/0000-0002-6464-7634)
- Paulo Afonso Granjeiro (ORCID: https://orcid.org/0000-0003-0322-0861)
- Yuri Abud (ORCID: https://orcid.org/0000-0002-3561-4817)
- José Antônio da Silva (ORCID: https://orcid.org/0000-0001-9134-1211)
Institutions
- Instituto Nacional de Metrologia, Qualidade e Tecnologia (BR)
- Federal University of São João del-Rei (BR)
Publication Details
- Journal
- Food Science and Technology
- Published
- 2025-05-14
- DOI
- https://doi.org/10.5327/fst.00423
- Primary Topic
- biodegradable polymer synthesis and properties
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
- Fundação de Amparo à Pesquisa do Estado de Minas Gerais
- Universidade Federal de São João del-Rei