PHYTOCHEMICAL PROFILING OF Tinospora rumphii STEMS CULTIVATED IN VARYING SOIL TYPES

This study aims to determine the effects of soil type on the phytochemical profiles (type and abundance) of Tinosphora rumphii stem ethanolic extract collected from Adtuyon Clay loam and hydrosol types of soil. The plant stem extract was concentrated using rotary evaporator until it is semi-solid in form. Phytochemical screening was done to evaluate the plant’s phytochemical constituents. Results showed the presence of alkaloids, flavonoids, steroids, and tannins in the T. rumphii extract obtained from two sites with Adtuyon Clay loam and hydrosol types of soil. On the other hand, saponins are present only in the plant sample obtained in Adtuyon type of soil. Results also revealed variations on the abundance of some phytochemicals present in T. rumphii stem collected from the two types of soil in which alkaloids and tannins are less in abundant in Adtuyon clay loam soil while high in abundance in hydrosol type. Hydrosol type of soil is effective in storing alkaloids and tannins in T. rumphii stem could be due to the presence of iron and manganese in Hydrosol type of soil that are necessary for accumulation of such phytochemicals in the plant tissues. On the other hand, Adtuyon clay loam soil is valuable in storing saponins.