Protocol for extracellular recordings of mechanically stimulated pentascolopidial chordotonal neurons in Drosophila larvae

Chordotonal organs are sensory structures containing ciliated neurons specialized in detecting mechanical forces. Here, we present a protocol for recording the neuronal activity of mechanically stimulated pentascolopidial chordotonal organs (lch5) of Drosophila larvae. We describe steps for dissecting larvae, measuring electrical activity of chordotonal neurons, and quantifying action currents. This protocol is useful for studying the biology of larval lch5 neurons, as well as the molecular mechanisms and components that contribute to their mechanosensitive properties. For complete details on the use and execution of this protocol, please refer to Bormann et al.¹.