Structural characterization and in vitro gut microbiota fermentation of a polysaccharide extracted from Cenostigma nordestinum gum
This study aims to structurally characterize a polysaccharide extracted from Cenostigma nordestinum gum (AGCn) and evaluate its prebiotic potential in human gut microbiota. AGCn, obtained by dissolution in water and precipitation in 70 % ethanol, was primarily composed of carbohydrates (93.61 %), with a low protein content (1.70 %) and some phenolic compounds (9.16 % Gallic Acid Equivalents). Its molecular weight was 6.19 × 10<sup>4</sup> g/mol, with a dispersity of 1.33. The main monosaccharides identified were arabinose (38 mol%) and galactose (31 mol%). Methylation analysis and 2D NMR spectra showed that the gum exuded from C. nordestinum is a branched arabinogalactan composed of →6)-β-Galp-(1→, →3,6)-β-Galp-(1→, and →3,4,6)-β-Galp-(1→ residues, containing chains of →3)-α-Araf-(1→ and →5)-α-Araf-(1→, with α-Araf-(1→ and α-Rhap-(1→ as terminal residues. The identification of →4)-α-GalpA-(1→ showed also the presence of a pectic polysaccharide. AGCn was metabolized by Lactobacillus brevis, Lactobacillus plantarum, and Lactobacillus casei probiotic bacteria. AGCn in vitro fermentation increased the ratio of Bifidobacterium and Bacteroides in the microbiota, decreased the abundance of bacteria associated with intestinal disease, and produced short-chain fatty acids. Thus, AGCn is an arabinogalactan with prebiotic potential as a gut microbiota modulator, offering significant value for future food and biotechnological applications.
Authors
- Marthyna Pessoa de Souza (ORCID: https://orcid.org/0000-0002-8629-143X)
- Francisca Crislândia Oliveira Silva (ORCID: https://orcid.org/0000-0002-0928-4690)
- Angélica T. Vieira (ORCID: https://orcid.org/0000-0002-4556-7671)
- Abigail González (ORCID: https://orcid.org/0000-0002-8178-0162)
- José António Teixeira (ORCID: https://orcid.org/0000-0002-4918-3704)
- Maria Isabela Ferreira de Araújo (ORCID: https://orcid.org/0000-0003-0908-4661)
- Maria Tereza dos Santos Correia (ORCID: https://orcid.org/0000-0003-4920-9975)
- Antonio Santos (ORCID: https://orcid.org/0000-0002-1253-585X)
- Paulo Rodinei Soares Lopes (ORCID: https://orcid.org/0000-0002-3005-4099)
- Thiago Barbosa Cahú (ORCID: https://orcid.org/0000-0002-7853-0613)
- Clarisse Nobre (ORCID: https://orcid.org/0000-0001-9693-2395)
- Soraia P. Silva (ORCID: https://orcid.org/0000-0002-6475-6689)
- Manuel A. Coimbra (ORCID: https://orcid.org/0000-0001-8898-6342)
- Andreia F. Salvador (ORCID: https://orcid.org/0000-0001-6037-4248)
- Elisabete Coelho (ORCID: https://orcid.org/0000-0002-2083-9634)
Institutions
- Universidade Federal de Minas Gerais (BR)
- Universidade Federal do Ceará (BR)
- Universidade Federal de Pernambuco (BR)
- Universidade Federal de Campina Grande (BR)
- Czech Academy of Sciences, Institute of Biophysics (CZ)
- Rede de Química e Tecnologia (PT)
- University of Aveiro (PT)
- University of Minho (PT)
Publication Details
- Journal
- Carbohydrate Polymers
- Published
- 2025-05-14
- DOI
- https://doi.org/10.1016/j.carbpol.2025.123738
- Citations
- 3
- Primary Topic
- Polysaccharides Composition and Applications
- Type
- article
- Field-Weighted Citation Impact
- 3.49
Funders
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
- Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco
- Universidade Federal de Pernambuco
- Rede de Química e Tecnologia
- Laboratório Associado para a Química Verde
- Fundação para a Ciência e a Tecnologia