Biological activity potential of Thalictrum orientale: antioxidant, anticholinesterase, and antiproliferative effects

Plants are among the important natural resources in terms of biological activity. In this study, the biological activity of Thalictrum orientale species was evaluated comprehensively. For this purpose, ethanol extract of the plant was obtained by Soxhlet extraction method. To determine the antioxidant capacity, total antioxidant status (TAS) and total oxidant status (TOS) were analyzed using Rel Assay kits. Anticholinesterase activity was determined by measuring acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzyme activities. In addition, antiproliferative activity was evaluated on A549 lung cancer cell line. As a result of the analyzes, TAS value of Thalictrum orientale was determined as 5.413±0.149 mmol/L, TOS value as 8.300±0.056 µmol/L and oxidative stress index (OSI) as 0.154±0.004. Within the scope of anticholinesterase activity, AChE inhibition value was determined as 35.41±1.88 µg/mL and BChE inhibition value was determined as 58.63±1.24 µg/mL. It was observed that the antiproliferative effect increased in direct proportion to the increase in concentration. The findings show that Thalictrum orientale has high biological potential in terms of antioxidant, antiproliferative and anticholinesterase activities.