Antithrombotic effect in zebrafish of a fibrinolytic protein EPF3 from Dilong (Pheretima vulgaris Chen) and its transport mechanism in Caco-2 monolayer through cell bypass pathway
EPF3 could play a thrombolytic and antithrombotic role in zebrafish. It could be transported and absorbed into the intestine through cellular bypass pathway by opening the intestinal epithelium tight junction. This study provides a scientific explanation for the antithrombotic effect of earthworm and provides a basis for the feasibility of subsequent development of EPF3 as an antithrombotic enteric-soluble preparation. Please cite this article as: Zhong WL, Yang JQ, Liu H, Wu YL, Shen HJ, Li PY, Du SY. Antithrombotic effect in zebrafish of a fibrinolytic protein EPF3 from Dilong (Pheretima vulgaris Chen) and its transport mechanism in Caco-2 monolayer through cell bypass pathway. J Integr Med. 2025; 23(4): 415-428.
Authors
- Wan-Ling Zhong (ORCID: https://orcid.org/0009-0004-5577-6065)
- Hai Liu (ORCID: https://orcid.org/0000-0002-7050-3880)
- Yali Wu (ORCID: https://orcid.org/0000-0001-7788-8130)
- Jianqiong Yang (ORCID: https://orcid.org/0000-0002-9063-8117)
- Pengyue Li (ORCID: https://orcid.org/0000-0003-3738-8662)
- Huijuan Shen (ORCID: https://orcid.org/0000-0002-3714-5348)
- Shouying Du (ORCID: https://orcid.org/0009-0004-0246-2409)
Institutions
- Beijing University of Chinese Medicine (CN)
- First Affiliated Hospital of Gannan Medical University (CN)
- First Affiliated Hospital of Henan University (CN)
Publication Details
- Journal
- Journal of Integrative Medicine
- Published
- 2025-05-14
- DOI
- https://doi.org/10.1016/j.joim.2025.05.003
- Primary Topic
- Signaling Pathways in Disease
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China
- National University's Basic Research Foundation of China
- Fundamental Research Funds for the Central Universities