Molecular Typing of Staphylococcus aureus Isolated from Canine Pyometra

Background: Pyometra is a chronic disease condition in bitches, characterised by the accumulation of pus within the uterus. Multi-drug resistant bacterial strains with biofilm-forming ability are one of the most significant reasons behind the failure of medical management. The present study was envisaged for the detection of antibiotic resistance, biofilm-forming ability and molecular typing of S. aureus isolated from canine pyometra. Methods: A total of 40 samples were collected from dogs with pyometra and following isolation and identification, DNA was extracted from the pure cultures of 13 coagulase positive Staphylococcus isolates. Result: Six isolates turned out to be Staphylococcus aureus in nuc gene specific PCR and in antibiogram, all were found to be multidrug resistant. Out of the 13 staphylococcal isolates, eight turned out be positive for the presence of mecA gene, which included five S. aureus isolates. Out of 13 isolates, 53.8% were found to be strong biofilm producers in tissue culture plate method. Among the 13 staphylococcal isolates, 53.8% showed the presence of icaD gene, whereas, 46.1% showed the presence of bap gene. All the six S.aureus isolates obtained in the study were subjected to agr typing by PCR and all belong to agr type I. All the isolates turned out to be belonging to spa type t037.