Isolation and Antimicrobial Screening of Actinomycetes spp. From Processed Mango Waste

Actinomycetes strains were isolated from mango Mangifera indica L. compost and yard waste.Actinomycetes strains were propagated in Bennett medium and later tested for antimicrobial and antifungal activity using the agar plug method.Strain A and strain C were isolated using the swirling method, and strain B was isolated by the spread plate method.Three of the compounds showed antagonistic activity against Gram-positive and Gram-negative bacteria and fungi.The colour of the substrate mycelium and aerial spore mass was varied.The three strains also showed positive results in biochemical tests such as protein hydrolysis, starch hydrolysis, catalase test, and negative results in nitrate reduction.The strains also showed a positive reaction in glucose fermentation in the triple sugar iron medium.Further purification and extraction of active substances are recommended, including the use of electron microscopy and molecular tools for strain characterization and identification.