Isolation and Diagnosis of Bacteria Contaminating the Hospital Environment

Objective: To find out which antibiotic has the greatest effectiveness against bacteria. Study Design: Cross sectional study Place and Duration of Study: This study was conducted at the Department of Biology, College of Education for Pure Sciences, University of Diyala, 32001, Baqubah, Iraq from 10th December to 31st December 2024. Methods: Thirty swabs were taken from beds and waste at Baqubah Teaching Hospital, Iraq and cultured on two types of media (blood agar and MacConkey agar). Five types of antibiotics (ciprofloxacin, amikacin, ampicillin, cefoxitin, and co-trimoxazole) were then applied to the growing bacteria. Results: Ciprofloxacin, Amikacin, Ampicillin, Cefoxitin and Co-Trimoxazol on Escherichia bacteria grown on molar Hinton agar where taken from hospital beds to show any antibiotics have the greatest effect on them. TheCiprofloxacin antibiotic was the best for killing Escherichia, with Inhibition zone diameter 1.25 cm, while Amikacin and Ampicillin killing Escherichia bacteria, with Inhibition zone diameter 1 cm, and the antibiotics Cefoxitin and Co-Trimoxazol, killing bacteria, with Inhibition zone diameter of 30 mm Conclusion: Ciprofloxacin antibiotic was the best for killing Escherichia bacteria, with inhibition zone diameter 1.25 cm, while Amikacin and Ampicillin kill Escherichia bacteria, with inhibition zone diameter 1 cm, and the antibiotics Cefoxitin and Co-Trimoxazol, killing bacteria, with inhibition zone diameter of 30 mm. Key Words: Hospital beds, Antibiotic, Escherichia coli