Paternal Lineage Detection of Pasundan Bulls Inferred from Partial Sex Region Y (SRY) Sequence

The Pasundan breed is native cattle from West Java, Indonesia, that the farmers keep as beef cattle.This study aimed to detect the paternal lineage of Pasundan bulls inferred from the partial sex determining region Y (SRY) gene.The blood samples of Pasundan bulls (20 heads) and frozen sperm (straw) of Ongole grade bulls (10 heads) were used for DNA extraction analysis.In the present study, Pasundan and Ongole grade bulls were kept at the breeding station.The results showed that a g.15264C>T mutation point was detected in the SRY gene of animals under study.This mutation was shown as the genetic marker to classify them into Indicine and Taurine lineages.In conclusion, Pasundan cattle in this study have two paternal lineages of Bos indicus (80%) and Bos taurus (20%).