Determination of antibodies to thyroid peroxidase: Further experiences

ABSTRACT: Introduction: Thyroid peroxidase autoantibodies are a very important diagnostic parameter. They are mainly found in patients with autoimmune thyroid diseases (Hashimoto's thyroiditis, spontaneous hypothyroidism, Bazedov's disease). Objective: Analysis of the results of the method we use in clinical practice. Material and working methods: Autoantibodies to thyrocytic peroxidase were determined using the Monobind method, USA. So far we have made over 2000 determinations. The method consists of a chemiluminescent immunoassay, using thyroid peroxidase labeled with biotin and plastic tubes coated with streptavidin. Calibration was done with international standard 66/387 for thyroid microsomes. Results: In the last 8 years, we have done over 2000 determinations with hundreds of people. In about fifty of them the examined parameter was done as a "follow-up" study. Conclusion: We presented the results of determination of antibodies to thyrocytic peroxidase. The method we used gives very reliable results. During the "follow-up" study, the results were sometimes not constant in the same subject.